Immunohistochemistry methods, that generally include an antigen retrieval step, have been optimised for formalin-fixed tissues, and tissue specimens can be stored in formalin for extended periods without major deleterious effects.
Molecular techniques such as ISH have also been validated for use on formalin fixed tissue. It is against these characteristics that a new or replacement fixative must be judged.
Some laboratories are currently looking to replace formalin with a less toxic reagent and there are several alternatives described in the previous paragraphs. In a research environment, where a specific tissue element is being studied, there may be more control over the fixation step, and it is worthwhile testing several reagents before making a final decision. If you are contemplating a change of fixative consider the following properties in addition to evaluating the results you see under the microscope:.
Changing to a different fixative requires very careful consideration and thorough evaluation. The content, including webinars, training presentations and related materials is intended to provide general information regarding particular subjects of interest to health care professionals and is not intended to be, and should not be construed as, medical, regulatory or legal advice.
Any links contained in the content which provides access to third party resources or content is provided for convenience only. For the use of any product, the applicable product documentation, including information guides, inserts and operation manuals should be consulted.
Leica Biosystems and the editors hereby disclaim any liability arising directly or indirectly from the use of the content, including from any drugs, devices, techniques or procedures described in the content. All rights reserved. Geoffrey Rolls is a Histology Consultant with decades of experience in the field. We are looking for more great writers to feature here. Send us a submission and we'll be in touch! Get more Knowledge Pathway content like this delivered directly to your inbox.
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Phosphate buffered formalin. Formal calcium. Formal saline. Zinc formalin unbuffered. Formulation Distilled water: ml Mercuric chloride: 50 g Potassium dichromate: 25 g Glacial acetic acid: 50 ml Fixation time: 4 — 24 hours Recommended Applications Gives good nuclear preservation but lyses red blood cells due to the presence of acetic acid.
Formulation Stock solution Mercuric chloride: 12 g Sodium acetate anhydrous: 2. Formulation Picric acid saturated aqueous solution. Fixation time: 4 — 18 hours Recommended Applications Recommended for gastrointestinal tract specimens and fixation of endocrine tissues.
Hazards: Bouin's solution contains formaldehyde more on formaldehyde. Bouin's solution also contains Picric acid which gives the solution its yellow color. Picric acid is highly explosive when dry. Recommendations: If you are not a frequent user of Bouin's solution, we recommend you consider purchasing small quantities of your fixative premade from any one of a number of suppliers. This reduces hazardous waste, removes the Picric Acid hazard and ensures fresh fixative.
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